Match details

Record ID: AF430711.1
Database: nt Go to original record
Description: Caesalpinia cassioides chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon: Caesalpinia cassioides
Original size: 1077 bp

Method: QL-PLN-00-007 Go to GMOMETHODS record
View in JRC GMO-Matrix
Description:Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method


Predicted amplification details:
Mismatches Gaps From To Strand Amplicon size
0 0 1 605 + 605 bp

Amplicon: 605 bp
1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggaaagg gagaataaaa aaaggatagg tgcagagact caacggaagc
201   tgttctaaca aatggagttg acgacatttt gtttcgttag taaaggaatc
251   cttccatcga aactccagaa aagaaaggat caaggatgaa catatatata
301   cgtactgaaa tactatttca attgattaag accagacaga ccccaaatct
351   ctatttttta atatttatat gacaaatgaa agatgtgaat agattccaag
401   ttgaagaaag aatcgaatat ttatatttat tgatcaaatc attcactcca
451   tcatagtctg atagatcttt tgaagaactg attaatcgga cgagaataaa
501   gatagagtcc cattctacat gtcaataccg acaacaatga aatttatagt
551   aagaggaaaa tccgtcgact ttagaaatcg tgagggttca agtccctcta
601   tcccc 

Primers alignment:
Primer/Probe % Identity Length Mismatches Gaps From To Score
primerA 100.0 20 bp 0 0 1 20 2.73e-08
primerB 100.0 20 bp 0 0 605 586 2.73e-08


Related publications:

Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101. 

Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.