JRC GMO-Amplicons | EURL GMFF

Record ID:	AF430709.1
Database:	nt	Go to original record
Description:	Caesalpinia cacalaco chloroplast tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer region, complete sequence; and tRNA-Phe (trnF) gene, partial sequence.
Taxon:	Caesalpinia cacalaco
Original size:	1091 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	597	+	597 bp

Amplicon:

597 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctaccaag tgagaacttt caaattcaga gaaaccctgg aattaacaat
101   gggcaatcct gagccaaatc ctgttttccg aaaaccaaga agagttcaga
151   aagggagaat aaaaaaggat aggtgcagag actcaacgga agctgttcta
201   acaaaaggag ttgacgacat ttcgtttcgt tagtaaagga atccttccat
251   cgaaactcca gaaaagaaag gatcaaggat gaacatatat atacgtatac
301   gtactgaaat actatttcaa ttgattagac cagacaaacc ccaaatctct
351   attttttaat atttatatga caaatgaaag atgtgaatag attccaagtt
401   gaagaaagaa tcaaatattt attgatcaaa tcattcactc catcatagtc
451   tgatagatct tttgaagaac tgattaatcg gacgagaata aagatagagt
501   cccattctac atgtcaatac cgacaacaat gaaatttata gtaagaggaa
551   aatccgtcga ctttagaaat cgtgagggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	597	578	2.69e-08

Match details