JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ874971.1
Database:	nt	Go to original record
Description:	Rytigynia xanthotricha chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Borhidi et al. 87353 (UPS)
Taxon:	Rytigynia xanthotricha
Original size:	1739 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	815	1371	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcataa
151   agtgaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaatgattaa tttaatgacg
301   cctcgactga atccgtattt tttatatgaa aacggaagaa ttggtgtgaa
351   tagattccac attgaagaaa gaatcgaata ttcattgatt aaatgattca
401   ctccatagtc tgatagatct tttcaagaat tgattaatcg gacgagaata
451   aagatagagt cccgttctac atgtcaatgt cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details