JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ874967.1
Database:	nt	Go to original record
Description:	Rytigynia seyrigii chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Gereau et al. 5731 (MO)
Taxon:	Rytigynia seyrigii
Original size:	1731 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	804	1354	+	551 bp

Amplicon:

551 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaagg gataggtgca gagactcaat ggaagttgtt ctaacaaatg
201   gagttggctg cgttagtaaa gaaatctttc catcgaaaat tccgaaagga
251   taaaacgtat acgtattgaa tactatatca aatgattaat gacgcctcga
301   ctgaatccgt attttttata tgaaaacgga agaattggtg tgaatagatt
351   ccacattgaa gaaagaatcg aatattcatt gattaaatga ttcactccat
401   agtctgatag atcttttcaa gaattgatta atcggacgag aataaagata
451   gagtcccgtt ctacatgtca atgtcggcaa caatgaaatt tatagtaaga
501   ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc cctctatccc
551   c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	551	532	2.48e-08

Match details