JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ874948.1
Database:	nt	Go to original record
Description:	Multidentia sclerocarpa chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Luke 9442 (UPS)
Taxon:	Multidentia sclerocarpa
Original size:	1729 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	805	1361	+	557 bp

Amplicon:

557 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    atccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa agcaaaggtt
151   cagaaaatga aaaagggata ggtgcagaga ctcgatggaa gttgttctaa
201   caaatggagt tggctgcgtt agtaaagaaa tctttccatc gaaaattccg
251   aaaggataaa acgtatacgt attgaatact atatcaaatg attaatgacg
301   actcgactga atccgtattt tttatatgaa aacggaagag ttggtgtgaa
351   tagattccac attgaagaaa gaatcgaata ttcattgatt aaatgattca
401   ctccatagtc tgatagatct tttcaagaat tgattaatcc gacgagaata
451   aagatagagt cccgttctac atgtcaatgt cggcaacaat gaaatttata
501   gtaagaggaa aatccgtcga ctttaaaaat cgtgagggtt caagtccctc
551   tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.51e-08
primerB	100.0	20 bp	0	0	557	538	2.51e-08

Match details