JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ874945.1
Database:	nt	Go to original record
Description:	Lagynias monteiroi chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Bremer et al. 4297 (UPS)
Taxon:	Vangueria monteiroi
Original size:	1735 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	807	1367	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcga tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacaaa cgtatacgta ttcaatacta tatcaaatga ttaatgacga
301   ctcgactgaa tccaatccgt attttttata tgaaaacgga agagttggtg
351   tgaatagatt ccacattgaa gaaagaatcg aatattcatt gattaaatga
401   ttcactccat agtctgatag atcttttcaa gaattgatta atcggacgag
451   aataaagata gagtcccgtt ctacatgtca atgtcggcaa caatgaaatt
501   tatagtaaga ggaaaatccg tcgactttaa aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details