JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ874944.1
Database:	nt	Go to original record
Description:	Fadogia triphylla chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Bidgood et al. 589 (UPS)
Taxon:	Fadogia triphylla
Original size:	1724 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	815	1366	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaac cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaaagg
251   ataaaacgta tacgtattga atactatatc aaatgattaa tgacgcctcg
301   actgaatccg tatttttkat atgaaaacgg aagagttggt gtgaatagat
351   tccacattga agaaagaatc gaatattcat tgattaaatg attcactcca
401   tagtctgata gatcttttca agaattgatt aatcggacga gaataaagat
451   agagtcccgt tctacatgtc aatgtcggca acaatgaaat ttatagtaag
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details