JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ874942.1
Database:	nt	Go to original record
Description:	Fadogia agrestis chloroplast trnT-trnL IGS (partial), tRNA-Leu (UAA) gene and trnL-trnF IGS (partial), specimen voucher Madsen 5495 (S)
Taxon:	Fadogia agrestis
Original size:	1722 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	807	1349	+	543 bp

Amplicon:

543 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcga tggaagttgt tctaacaaat
201   ggagttggct gcgttagtaa agaaatcttt ccatcgaaaa ttccgaacgt
251   atacgtattg aatactatat caaatgatta atgacgactc gactgaatcc
301   gtatttttta tatgaaaacg gaagagttgg tgtgaataga ttccacattg
351   aagaaagaat cgaatattca ttgattaaat gattcactcc atagtctgat
401   agatcttttc aagaattgat taatcggacg agaataaaga tagagtcccg
451   ttctacatgt caatgtcggc aacaatgaaa tttatagtaa gaggaaaatc
501   cgtcgacttt aaaaatcgtg agggttcaag tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	543	524	2.44e-08

Match details