JRC GMO-Amplicons | EURL GMFF

Record ID:	HM369421.1
Database:	nt	Go to original record
Description:	Loropetalum chinense isolate L-Hap_1 tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Loropetalum chinense
Original size:	588 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	588	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattt gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaaaaag
101   gggcaatcct gagccaaatc ctgttttccg aaaacaaaga caagggttca
151   gaaagcgaga atcaaaataa aaaaaggata ggtgcagaga ctcaatggaa
201   gctgttctaa caaatggagt tgactgcgtt acgttggtaa aggaatcttt
251   ttatggaaac tccagaaagg atgaaggata aacctatata catacgtata
301   cgtactaaaa tactatatca actgattaag gacgattcga atctgtattt
351   tttatatgaa aaatgaaaaa attgttgtga atcgattcca agttgaagaa
401   agaatcaaat attcattgat aaaataattc actccatggt ctgatagatc
451   ttttgaagat ctgattaatc ggacgagaat aaagatagag tcccattcta
501   catgtcaatg ccgacaacaa tgaaatttat agtaagagga aaatccgtcg
551   actttagaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details