JRC GMO-Amplicons | EURL GMFF

Record ID:	JF796859.1
Database:	nt	Go to original record
Description:	Cypripedium reginae isolate C007 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cypripedium reginae
Original size:	626 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	609	+	608 bp

Amplicon:

608 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaaat
101   gggcaatcct gagccaaatc cttttttttt atggaaaaag ggataggtgc
151   agagactcaa tggaagctgt tctaacgaat gaaattgact acgttacgtt
201   agtagcaaaa atccttctat cgaaatgata gaaatgacag taaaggataa
251   gcttatatac ctaatacata ctgacatagg aaacgattaa tcacaacacg
301   aatcctatat ctctaataat attttaatat ttagtaataa ctagtaatat
351   attctaatta gtttctaatt attatttcat aatattctaa taatattata
401   atacttaatt tagaatactt aatactatat actattcata ataatgatat
451   gagtattagt atgagtatat agatcttttg aaagttaatc ggacgagaat
501   aaagagagag tcccatttta catgtcaata ccgacaacaa tgaaatttat
551   agtaagagga aaatccgtcg acttttaaaa tcgtgagggt tcaagtccct
601   ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.74e-08
primerB	100.0	20 bp	0	0	608	589	2.74e-08

Match details