JRC GMO-Amplicons | EURL GMFF

Record ID:	JF796858.1
Database:	nt	Go to original record
Description:	Cypripedium passerinum isolate C006 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Cypripedium passerinum
Original size:	624 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	607	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttgattg gattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aactaaaatg
101   agcaatctga gccaaatcct ttttttttat ggaaaaaggg ataggtgcag
151   agactcaatg gaagctgttc taacgaatga aattgactac gttacgttag
201   tagcaaaaat ccttctatcg aaatgataga aatgacagta aaggataagc
251   ttatatacct aatacatact gacataggaa acgattaatc acaacacgaa
301   tcctatatct ctaataatat tttaatattt agtaataact agtaatatat
351   tctaattagt ttctaattat tatttcataa tattctaata atattataat
401   acttaattta gaatacttaa tactatatac tattcataat aatgatatga
451   gtattagtat gagtatatag atcttttgaa agttaatcgg acgagaataa
501   agagagagtc ccattttaca tgtcaatacc gacaacaatg aaatttatag
551   taagaggaaa atccgtcgac ttttaaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details