Match details
Record ID: |
JN400383.1 |
Database: |
nt |
Go to original record |
Description: |
Expression vector pMON93914, complete sequence. |
Taxon: |
Expression vector pMON93914 |
Original size: |
11397 bp |
View other 13 matches for this record
Method: |
QL-CON-00-008 |
Go to GMOMETHODS record |
|
View in JRC GMO-Matrix
|
Description: | Qualitative PCR method for detection of the junction between the chloroplast transit peptide 2 and the CP4 epsps gene (Grohmann et al., 2009). |
Target: | CTP2-CP4 EPSPS |
View other 257 matches for this method
Predicted amplification details: |
Mismatches |
Gaps |
From |
To |
Strand |
Amplicon size |
0 |
0 |
8479 |
8566 |
- |
88 bp |
|
1 gggatgacgt taattggctc tgagcttcgt cctcttaagg tcatgtcttc
51 tgtttccacg gcgtgcatgc ttcacggtgc aagcagcc
View other 233 matches with an identical amplicon
Primers and probe alignment: |
Primer/Probe |
% Identity |
Length |
Mismatches |
Gaps |
From |
To |
Score |
primerA |
100.0 |
22 bp |
0 |
0 |
1 |
22 |
3.82e-10 |
primerB |
100.0 |
19 bp |
0 |
0 |
88 |
70 |
1.47e-08 |
probe |
100.0 |
27 bp |
0 |
0 |
65 |
39 |
8.22e-13 |
|
Related publications:
Petrillo, M., et al. “JRC GMO-Amplicons: A Collection of Nucleic Acid Sequences Related to Genetically Modified Organisms.” Database (Oxford), vol. 2015 bav101, 30 Sep. 2015, doi:10.1093/database/bav101.
Angers-Loustau, A., et al. “Using the EURL GMFF Online Bioinformatics Resources: A how-to Guide for Practical JRC GMO-Matrix and JRC GMO-Amplicons Case Uses.” European Commission, Ispra, JRC101853, 2016.