JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ382018.1
Database:	nt	Go to original record
Description:	Babiana torta tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Babiana torta
Original size:	773 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	548	+	547 bp

Amplicon:

547 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aaaaaaaagg
101   gggcaatcct gagccaaatc tttattttga gaaaacaaaa actagaataa
151   aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa cgaatggagt
201   tgactacgtt gtgttggtag ccggaatctt tctatcgaaa tgaaagataa
251   ggatatacct aattcgtata catattacca tatcaaacga ttaatcatga
301   cccaaatcca ttatatatat atgcaaaatg ggggagatct attccaatcg
351   agaagaatcg aatattcggt gatcaaatct attctataca ttccagggtt
401   tgatagatct tttgaaaaac agattaatcg gaagagaata aagagagagt
451   cccattatac atgtcaataa cgacaacaat gaaatttata gtaaaaggaa
501   aatccgtcga ctttagaaat cgtgakggtt caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.46e-08
primerB	100.0	20 bp	0	0	547	528	2.46e-08

Match details