JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ381970.1
Database:	nt	Go to original record
Description:	Babiana curviscapa tRNA-Leu (trnL) gene, partial sequence; chloroplast.
Taxon:	Babiana curviscapa
Original size:	550 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	550	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aaaaaaaaaa
101   gggggcaatc ctgagccaaa tctttatttt gagaaaacaa aaactagaat
151   aaaaaaagga taggtgcaga gactcaatgg aagctgttct aacgaatgga
201   gttgactacg ttgtgttggt agccggaatc tttctatcga aatgaaagat
251   aaggatatac ctaattcgta tacatattac catatcaaac gattaatcat
301   gacccaaatc cattatatat atatgcaaaa tgggggagat ctattccaat
351   cgagaagaat cgaatattcg gtgatcaaat ctattctata cattccaggg
401   tttgatagat cttttgaaaa acagattaat cggaagagaa taaagagaga
451   gtcccattat acatgtcaat aacgacaaca atgaaattta tagtaaaagg
501   aaaatccgtc gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details