JRC GMO-Amplicons | EURL GMFF

Record ID:	GQ381962.1
Database:	nt	Go to original record
Description:	Babiana attenuata tRNA-Leu (trnL) gene, partial sequence; trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Babiana attenuata
Original size:	746 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	549	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttgattg aattgagcct tagtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aaaaaaaaag
101   ggggcaatcc tgagccaaat ctttattttg agaaaacaaa aactagaata
151   aaaaaaggat aggtgcagag actcaatgga agctgttcta acgaatggag
201   ttgactacgt tgtgttggta gccggaatct ttctatcgaa atgaaagata
251   aggatatacc taattcgtat acatattacc atatcaaacg attaatcatg
301   acccaaatcc attatatata tatgcaaaat gggggagatc tattccaatc
351   gagaagaatc gaatattcgg tgatcaaatc tattctatac attccagggt
401   ttgatagatc ttttgaaaaa cagattaatc ggaagagaat aaagagagag
451   tcccattata catgtcaata acgacaacaa tgaaatttat agtaaaagga
501   aaatccgtcg actttagaaa tcgtgakggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details