JRC GMO-Amplicons | EURL GMFF

Record ID:	AY836545.1
Database:	nt	Go to original record
Description:	Menodora scabra tRNA-Leu (trnL) and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Menodora scabra
Original size:	915 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	562	+	552 bp

Amplicon:

552 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctgctaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aaagggcaat cctgagccaa atccttcccc aaacaaaagt tcagaaagaa
151   aaaagggata ggtgcagaga ctcaatggaa gctgttctaa gaaatggagt
201   ggactgcgtt ggtagagtaa tctttccatc aaaacttcag aaaggatgaa
251   ggataactag tctagattta ctgctatatc aaatagcaaa tgattaatga
301   cagctcgaat ctctatctgt atttttttct atgaaaaatg gaagattgat
351   tccattttga agaaagaatc aaatattcat tgatcaaatc attcactcca
401   tagtctgata gatcttttaa aaaatggatt aatcggacga gaataaagat
451   agagtcccat tctacatgtc aataccggca acaatgaaat ttctagtaaa
501   aggaaaatcc gtcgacttta aaaatcgtga gggttcaagt ccctctatcc
551   cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.48e-08
primerB	100.0	20 bp	0	0	552	533	2.48e-08

Match details