JRC GMO-Amplicons | EURL GMFF

Record ID:	AY836543.1
Database:	nt	Go to original record
Description:	Jasminum leratii tRNA-Leu (trnL) and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Jasminum leratii
Original size:	958 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	560	+	550 bp

Amplicon:

550 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccccaaacaa gagttcagaa
151   agaaataaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagtggact aggttggtag aggaatcttt ccatcgaaac ttcagaaagg
251   atgaaggata aattgtatat attgaatact atatcaaatg attaatgacg
301   actcgaatct ctatctgtat ttttttatat gaaaaatgga agattgattc
351   cattttgaag aaagaatcga atattcattg atcaaatcat tcactccaca
401   gtctgataga tcttttaaag aactgattaa tcggacgaga ataaagatag
451   agtcccattc tacatgtcaa taccggcaat aatgaaattt atagtaaaaa
501   gaaaatccgt cgactttaaa aatcgtgagg gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	550	531	2.47e-08

Match details