JRC GMO-Amplicons | EURL GMFF

Record ID:	AY836541.1
Database:	nt	Go to original record
Description:	Jasminum floribundum tRNA-Leu (trnL) and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Jasminum grandiflorum subsp. floribundum
Original size:	932 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	559	+	549 bp

Amplicon:

549 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccccaaacaa gagttcagaa
151   agaaaaaagg gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagtggacta ggttggtaga ggaatctttc catcgaaact tcagaaagga
251   tgaaggataa attgtatata ttgaatacta tatcaaatga ttaatgacga
301   ctcgaatctc tatctgtatt tttttatatt caaaatggaa gattgattcc
351   attttcaaga aagaatcgaa tattcattga tcaaatcatt cactccacag
401   tctgatagat cttttaaaga actgattaat cggacgagaa taaagataga
451   gtcccattct acatgtcaat accggcaaca atgaaattta tagtaaaagg
501   aaaatccgtc gactttaaaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	549	530	2.47e-08

Match details