JRC GMO-Amplicons | EURL GMFF

Record ID:	AY836536.1
Database:	nt	Go to original record
Description:	Jasminum fruticans tRNA-Leu (trnL) and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Chrysojasminum fruticans
Original size:	949 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	584	+	574 bp

Amplicon:

574 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccccaaacaa aagttcagaa
151   agaaaaaaag gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagtgaacta cgttggtaga ggaatctttc catcgaaact tcagaaaaga
251   tgaaggataa attgtatata ttgaatacta tatctactat atcaaagaat
301   actatatcaa atgattaatg acgactcgaa tctctatctg tatttttttt
351   atatgaaaaa tggaagattg attgcatttt gaagaaagaa tcgaatatcc
401   attgatcaaa tcattcactc catagtctga tagatctttt aaagaactga
451   ttaatcggac gagaataaag atagagtccc gttctacatg tcaataccgg
501   caacaatgaa atttatagta aaaggaaaat ccgtcgactt taaaaatcgt
551   gagggttcaa gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	574	555	2.58e-08

Match details