JRC GMO-Amplicons | EURL GMFF

Record ID:	AY836535.1
Database:	nt	Go to original record
Description:	Jasminum multiflorum tRNA-Leu (trnL) and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Jasminum multiflorum
Original size:	957 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	558	+	548 bp

Amplicon:

548 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tggtaacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ccccaaacaa gagttcagaa
151   agaaaaaagg gataggtgca gagactcaat ggaagctgtt ctaacaaatg
201   gagtggacta ggttggtaga ggaatctttc catcgaaact tcagaaagga
251   tgaaggataa attgtatata ttgaatacta tatcaaatga ttaatgacga
301   ctcgaatctc tatctgtatt ttttatatga aaaatggaag attgattcca
351   ttttgaagaa agaatcgaat attcattgat caaatcattc actccacagt
401   ctgatagatc ttttaaagaa ctgattaatc ggacgagaat aaagatagag
451   tcccattcta catgtcaata ccggcaataa tgaaatttat agtaaaaaga
501   aaatccgtcg actttcaaaa tcgtgagggt tcaagtccct ctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.47e-08
primerB	100.0	20 bp	0	0	548	529	2.47e-08

Match details