JRC GMO-Amplicons | EURL GMFF

Record ID:	HM164338.1
Database:	nt	Go to original record
Description:	Trailliaedoxa gracilis voucher Yu 1348 (HUH) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Trailliaedoxa gracilis
Original size:	1674 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	764	1328	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcctgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaag ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttgact gcgttagtat agaaatcttt tcatcgaaaa ttccgaaagg
251   ataaagtcaa agataaacgt atatacatat tgaatactat atcaaatgat
301   taatgacgac tcgactgaat ctgtattttt tatatgaaaa cggaagaatt
351   ggtgtgaata aattccacat tgaagaaaga atcgaatatt cattgattac
401   atgattcact ccacagtctg atatattttt tcaagaattt attaatcgga
451   cgagaataaa gatagagtcc cgttctacat gtcaatgtcg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact tttcaaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details