JRC GMO-Amplicons | EURL GMFF

Record ID:	HM164329.1
Database:	nt	Go to original record
Description:	Mitriostigma axillare voucher Bremer 2705 (UPS) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Mitriostigma axillare
Original size:	1214 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	281	849	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   taaggggcaa tcctgagcca aatcccgttt tcccaaacca aaggttcaga
151   aagtgaaaaa gggataggtg cagagactca acggaagctg ttctaacaaa
201   tggagttggc tgcgttagta gagaaatctt tccatctaaa attccgaaag
251   gataaagtga aggataaacg tatatatacg tattgaatac tatatcaaat
301   gattaatgac gactcgactt aatctgtatt ttttatatga aaacggaaga
351   attggtgtga atagattcca cattgaagaa agaatcgaat attcattgat
401   caaatgattc actccatagt ctgatagatc ttttcaagaa ttgattaatc
451   ggatgagaat aaagatagag tcccgttcta catgtcaatg tcggcaacaa
501   tgaaatttat agtaagagga aaatccgtcg actttaaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details