JRC GMO-Amplicons | EURL GMFF

Record ID:	HM164311.1
Database:	nt	Go to original record
Description:	Bertiera longithyrsa voucher Karehed et al. 256 (UPS) tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Bertiera longithyrsa
Original size:	1715 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	784	1352	+	569 bp

Amplicon:

569 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct gcgttagtag agaaatcttt tcatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaatg
301   attaatgacg actcaactta atctgtattt tttttatata aaaacggaag
351   aattggtgtg aatagattcc acattgaaga aagaatcgaa tattcattga
401   tcaaatgatt cactccatag tctgatagat cttttcaaga attgattaat
451   cggacgagaa taaagataga gtcccgttct acatgtcaat gtcggcaaca
501   atgaaattta tagtaagagg aaaatccgtc gactttaaaa atcgtgaggg
551   ttcaagtccc tctatcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.56e-08
primerB	100.0	20 bp	0	0	569	550	2.56e-08

Match details