JRC GMO-Amplicons | EURL GMFF

Record ID:	HM164308.1
Database:	nt	Go to original record
Description:	Atractocarpus balansaeanus voucher Munzinger & McPherson 635 (MO) tRNA-Thr (trnT) gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu (trnL) gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe (trnF) gene, partial sequence; chloroplast.
Taxon:	Atractocarpus balansaeanus
Original size:	1692 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	823	1395	+	573 bp

Amplicon:

573 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    acccactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcagaa
151   agtgaaaaaa ggataggtgc agagactcaa cggaagctgt tctaacaaat
201   ggagttggct acgttagtag agaaatcttt ccatctaaaa ttccgaaagg
251   ataaagtgaa ggataaacgt atatatacgt attgaatact atatcaaaat
301   caaatgatta atgacgactc gactgaatct gtatttttta tatgaaaacg
351   gaagaattgg tgtgaataga ttccacattg aagaaagaat cgaatattca
401   ttgatcaaat gattcactcc atagtctgat agatcttttc aagaattgat
451   taatcgggcg agaataaaga tagagtcccg ttctacatgt caatgtcggc
501   aacaatgaaa tttatagtaa gaggaaaatc cgtcgacttt aaaaatcgtg
551   agggttcaag tccctctatc ccc

View other 4 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.58e-08
primerB	100.0	20 bp	0	0	573	554	2.58e-08

Match details