JRC GMO-Amplicons | EURL GMFF

Record ID:	AY651842.1
Database:	nt	Go to original record
Description:	Chusquea coronalis tRNA-Leu gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Chusquea coronalis
Original size:	1003 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	616	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc catgttttga gaaaacaagt ggttctcgaa
151   ctagaatcca aaggaaaagg ataggtgcag agactcaatg gaagctgttc
201   taacgaatcg agttgattac gttgtgttgg tagtggaact ccctctaaat
251   tagagaaagt aaggggttta tacatctaat acacactact gacatagcaa
301   acgattaatc acagaaccca taacataata taggttcttt attctttttt
351   agaatgaaat taggaatgat tttgaaatag aaaattcaga atttttttgg
401   aattattttg aatccattcc aatctaatat tgaataatca aatccttcaa
451   ttcttagttt tcgagatctt ttaaaaagtg gattaatcag acgaggataa
501   agagagagtc ccattctaca tgtcaatact gacaacaatg aaatttctag
551   taaaaggaaa atccgtcgac tttataagtc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details