JRC GMO-Amplicons | EURL GMFF

Record ID:	HM164304.1
Database:	nt	Go to original record
Description:	Airosperma trichotomum voucher Degener & Ordonez 15542 (S) trnT-trnL intergenic spacer, partial sequence; tRNA-Leu (trnL) gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Airosperma trichotomum
Original size:	1702 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	782	1351	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg aattgagcct tggtatggaa
51    atacactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aaggggcaat cctgagccaa atcccgtttt ccgaaaccaa aggttcaaaa
151   agggaaaaag gataggtgca gagactcaac ggaagctgtt ctaacaaatg
201   gagttggttg tgttagtaga gaaatctttc catcgaaaat tccgaaagga
251   aaaggtgaag gataaacgta tatatgtatt gaatgctata tcaaaagatt
301   aatgacgact cggttgaatc tgtatttttt atttttatat gaaaacagaa
351   gaattggtat gaatagattc cacattgaag aaagaatcga atattcatta
401   atcaaatgat tcactccata gtctgataga tcttttcaag aattgattaa
451   tcggacgaga ataaagatag agtcccgttc tacatgtcaa tgttggcaag
501   aatgaaattt atagtaagag gaaaatccgt cgactttaaa aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details