JRC GMO-Amplicons | EURL GMFF

Record ID:	HM536959.1
Database:	nt	Go to original record
Description:	Smilax china plastid, complete genome.
Taxon:	Smilax china
Original size:	157878 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46382	46996	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgatta gcttgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccccgg aattcaaaat
101   gggcaatcct gagccaaatc tttattttga taaaataggg gtttaattta
151   taaaactaga atcaaaaagg gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatg gagttgacta cgttacgttg cgttggtagc tggaatccct
251   ctatcaaaat tacagatagg atggccctat atacgtatat atacatactg
301   acatatcaaa cgattgatta attatgaata tatttatata tatatatata
351   tatatgatat gaatatatga atatgaaaaa aaaaaattga gagttattgt
401   gaatctattc caatcgaagt tgaaaaaaaa atcgaatatt cagttatcaa
451   attattcatt ccagagtctg aaaaatcttt tgaaaaactg attaattgga
501   cgaaaataaa gagagagtcc cattctacat gtcaatatcg acaacaatga
551   aatttatagt aaaaggaaaa tccgtcgact ttaaaagtcg ggggggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details