JRC GMO-Amplicons | EURL GMFF

Record ID:	GU592211.1
Database:	nt	Go to original record
Description:	Microlaena stipoides chloroplast, partial genome.
Taxon:	Microlaena stipoides
Original size:	134551 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46559	47173	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg ttttgagcct tggtatggaa
51    acctactaag tgttaacttc caaattcaga gaaaccctgg aattaaaaat
101   ggggcaatcc tgagccaaat ccttgttttg agaaaactag tggttctcga
151   actagaatcc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg gtagtggaac tctctnnnnn
251   nnnnnngaaa gaagggcttt atacatctaa tacacacgta tagatactga
301   catagcaaac gattaatcac agaactcata tcataatata tgttctttaa
351   ttctttttta gaatgcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
401   nnnnnnnnnn nnnnnnnnnn nnnnattcat atcgaatatt gagtaatcaa
451   atccttcaat tcatagtttt tgagatcttt taaaaagtgg attaatcgga
501   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaatga
551   aatttctagt aaaaggaaaa tccgtcgact ttataagttg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details