JRC GMO-Amplicons | EURL GMFF

Record ID:	GU592210.1
Database:	nt	Go to original record
Description:	Potamophila parviflora chloroplast, partial genome.
Taxon:	Potamophila parviflora
Original size:	134551 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	46559	47173	+	615 bp

Amplicon:

615 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   ggggcaatcc tgaaccaaat ccgtgttttg agaaaacaag tggttctcga
151   actagaatcc aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg gtagtggaac tcccnnctaa
251   attagagaaa gaagggcttt ctaaatctaa tacacacgta tagatactga
301   catagcaaac gattaatcac agaactcata tcataatata ggttctttaa
351   ttctttttta gaattaaaat aggaatgatt atgaaataaa aaattctgaa
401   tttttttaga gttattgtga atccattcca atcgaatatt gagtaatcaa
451   atccttcaat tcatagtttt cgaaatcttt taaaaagtgg attaatcgga
501   cgaggataaa gagagagtcc cattctacat gtcaatactg acaacaaaga
551   aatttctagt aaaaggaaaa tccgtcgact ttctaagtcg tgagggttca
601   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.77e-08
primerB	100.0	20 bp	0	0	615	596	2.77e-08

Match details