JRC GMO-Amplicons | EURL GMFF

Record ID:	GU323356.1
Database:	nt	Go to original record
Description:	Solanum villosum tRNA-Leu (trnL) gene, trnL-trnF intergenic spacer, and tRNA-Phe (trnF) gene, complete sequence; chloroplast.
Taxon:	Solanum villosum
Original size:	1057 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	11	573	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acttactaag tgatcacttt caaattcaga gaaaccctgg aattaacaaa
101   aatgggcaat cctgagccaa atcctgtttt ctgaaaacaa acaaaggttc
151   agaaaaaaag gataggtgca gagactcaat ggaagttatt ctaacaaatg
201   gagttaaatg cgttggtaga ggactcttta catcgaaact tcagaaagaa
251   aaagaatgaa gtgaaggata aacgtatatc catacgtatt gaatactata
301   tcaaatgatt aatgacgacc cgaatccgta gtttttcgat aaaaaatcga
351   agaattggtg tgaatccatt ctacattgaa gaaagaatcg aatattcatt
401   gatcaaatca ttcactccat agtctgatag atcaactgat taatcggacg
451   agaataaaga tagagtcccg ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt aaaaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details