JRC GMO-Amplicons | EURL GMFF

Record ID:	AY589134.1
Database:	nt	Go to original record
Description:	Poa ligulata voucher PI 517033 trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Poa ligulata
Original size:	1837 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	876	1438	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aattaaaaaa
101   gggcaatcct gagccaaatc cgtgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtggaat tcctttaata
251   aacacgtatc gatacttaca tagcaaacga ttaatcacag aacccatatt
301   ataatattgg ttctttattc ttttttagaa tgaaataaaa aattcagaat
351   ttttttcgaa ttattgtgaa tccattccaa tcgaatattt agtaatcaaa
401   tccttcaatt caaagttttg aggtctttaa aaaagtggat taatcggacg
451   aggacaaaga gagagtccca ttctacatgt caatactgac aacaatgaaa
501   tttctagtaa aaggaaaatc cgtcgacttt ataagtcgtg agggttcaag
551   tccctctatc ccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details