JRC GMO-Amplicons | EURL GMFF

Record ID:	AY589123.1
Database:	nt	Go to original record
Description:	Poa annua trnT-trnL intergenic spacer, partial sequence; tRNA-Leu gene, complete sequence; and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Poa annua
Original size:	1820 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	824	1439	+	616 bp

Amplicon:

616 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aataaaaaaa
101   gggcaatcct gagccaaatc catgttttga gaaaacaagg gggttctcga
151   actagaatac aaaggaaaag gataggtgca gagactcaat ggaagctgtt
201   ctaacgaatc gagttaatta cgttgtgttg ttagtataat tccttctaaa
251   ttcgaaaaag aagggcttta tacatctaat aaacacgtat cgatactgac
301   atagcaaacg attaatcaca gaacccatat tataatattg gttctttatt
351   cttttttaga atgaaataga aatgattatg aaataaaaaa ttctgcattt
401   ttttagaatt attatattgt gaatccattc caatcgaata tttagtaatc
451   aaatccttca attcaaagtt ttgaggtctt taaaaaagtg gattaatcgg
501   acgaggacaa agagagagtc ccattctaca tgtcaatact gacaacaatg
551   aaatttctag taaaaggaaa atccgtcgac tttataagtt gtgagggttc
601   aagtccctct atcccc

View other 26 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.78e-08
primerB	100.0	20 bp	0	0	616	597	2.78e-08

Match details