JRC GMO-Amplicons | EURL GMFF

Record ID:	AY523507.1
Database:	nt	Go to original record
Description:	Eleusine multiflora clone KH258 tRNA-Thr gene, partial sequence; trnT-trnL intergenic spacer, tRNA-Leu gene, and trnL-trnF intergenic spacer, complete sequence; and tRNA-Phe gene, partial sequence; chloroplast.
Taxon:	Eleusine multiflora
Original size:	1895 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	872	1492	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttgattg tattgagcct tggtatggaa
51    acctgctaag tggtaacttc caaattcaga gaaaccctgg aatgaaaaat
101   gggcaatcct gagccaaatc cctgttttta aaacaagtgg ttctcaaact
151   agaacccaaa ggaaaaggat aggtgcagag actcaatgga agctgttcta
201   acgaatcgag gtgtaattac gttgtgttgg tagtgaaact ccctctaaat
251   tactaaatta gagaaagaag agctttatac atctaataca cacgtataga
301   tactgacata gcaaacgatt aatcacagaa cccatactat aatataggtt
351   ctttatttat tttttagaat gaaatttgga atgattatga aatagaaaat
401   tctgaatttt tttagaatta ttgtgaatct attcctctcg aatattgagt
451   aatcaaatcc ttcaattcat tgtttttgag atctttttaa aataggatta
501   atcggacgag gataaagaga gagtcccatt ctacatgtca atactgacaa
551   caatgaaatt tctagtaaaa ggaaaatccg tcgactttat aagtcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details