JRC GMO-Amplicons | EURL GMFF

Record ID:	HM100614.1
Database:	nt	Go to original record
Description:	Stewartia sinensis isolate 8019 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Stewartia sinensis
Original size:	1009 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	587	+	586 bp

Amplicon:

586 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaatcct tccatcgaaa
251   cttcagaaag gatgaaagag aaacctatat acatacgcat acgtactgaa
301   atactatatc aaatgattaa tgacgaccca aatctgtatc cgtatttttt
351   tttatgaaaa atggaagaat tgttgtgaat cgattccacg ttgaataaag
401   aattgaatat tcattgatca aatcatttac tccatagttt gatagatctt
451   ttgaagaact gattaatcgg acgagaataa agatagagtc ccattctaca
501   tgtcaatacc gacaacaatg aaatttatag taagaggaaa atccgtcgac
551   tttagaaatc gtgagggttc aagtccctct atcccc

View other 2 matches with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.64e-08
primerB	100.0	20 bp	0	0	586	567	2.64e-08

Match details