JRC GMO-Amplicons | EURL GMFF

Record ID:	HM100603.1
Database:	nt	Go to original record
Description:	Schima argentea isolate 5890 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Schima argentea
Original size:	969 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	599	+	598 bp

Amplicon:

598 bp

1     cgaaatcggt agacgctacg gacttaatta gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   agaaagcgaa aatcaaaaaa ggataggtgc agagactcaa tggaagctgt
201   tctaacaaat ggagttgact gcgttggtag aggaatcctt ccatcgaaac
251   ttcagacgaa acttcagaaa ggatgaaaga gaaacctata tacatacgca
301   tacgtactga aatactatat caaatgatta atgacgaccc aaatctgtat
351   ccgtattttt tttatatgaa aaatggaaga attgttgtga atcgattcca
401   cgttgaataa agaattgaat attcattgat caaatcattt actccatagt
451   ttgatagatc ttttgaagaa ctgattaatc ggacaagaat aaagatagag
501   tcccattcta catgtcaata ccgacaacaa tgaaatttat agtaagagga
551   aaatccgtcg actttagaaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.69e-08
primerB	100.0	20 bp	0	0	598	579	2.69e-08

Match details