JRC GMO-Amplicons | EURL GMFF

Record ID:	HM100594.1
Database:	nt	Go to original record
Description:	Stewartia laotica isolate 4812 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Stewartia laotica
Original size:	1012 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	2	590	+	589 bp

Amplicon:

589 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa aaggataggt gcagagactc aatggaagct
201   gttctaacaa atggagttga ctgcgttggt agaggaatcc ttccaccgaa
251   acttcagaaa ggatgaaaga gaaacctata tacatacgca tacgtactga
301   aatactatat caaatgatta atgacgaccc aaatctgtat ccgtattttt
351   ttttttatga aaaatggaag aattgttgtg aattgattcc acgttgaata
401   aagaattgaa tattcattga tcaaatcatt tactccatag tttgatagat
451   cttttgaaaa actgattaat cggacgagaa taaagataga gtcccattct
501   acatgtcaat accgacaaca atgaaattta tagtaagagg aaaatccgtc
551   gactttagaa atcgtgaggg ttcaagtccc tctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	589	570	2.65e-08

Match details