JRC GMO-Amplicons | EURL GMFF

Record ID:	HM100592.1
Database:	nt	Go to original record
Description:	Stewartia cordifolia isolate 4564 tRNA-Leu (trnL) gene and trnL-trnF intergenic spacer, partial sequence; chloroplast.
Taxon:	Stewartia cordifolia
Original size:	1010 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	1	588	+	588 bp

Amplicon:

588 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccctgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt tcgaaaacaa acaaagattc
151   agaaagcgaa aataaaaaaa aggataggtg cagagactca atggaagctg
201   ttctaacaaa tggagttgac tgcgttggta gaggaatcct tccatcgaaa
251   cttcagaaag gatgaaagag aaacctatat acatacgcat acgtactgaa
301   atactatatc aaatgattaa tgacgaccca aatctgtatc cgtatttttt
351   tttttatgaa aaatggaaga attgttgtga atcgattcca cgttgaataa
401   agaattgaat attcattgat caaatcattt actccatagt ttgatagatc
451   ttttgaagaa ctgattaatc ggacgagaat aaagatagag tcccattcta
501   catgtcaata ccgacaacaa tgaaatttat agtaagagga aaatccgtcg
551   actttagaaa tcgtgagggt tcaagtccct ctatcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.65e-08
primerB	100.0	20 bp	0	0	588	569	2.65e-08

Match details