JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621123.1
Database:	nt	Go to original record
Description:	Lyperia antirrhinoides chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial)
Taxon:	Lyperia antirrhinoides
Original size:	1703 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	774	1338	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggtaat cctgagccaa atcctgtttt ctcaaaacaa aggttcataa
151   ggcgaaaaag ggataggtgc agagactcaa cggaagctgt tctaagaaat
201   ggagttgact gcgttaatag aagaatcttt ccatcgaaac ttcagaaagg
251   gtggcggata aacgtatcta ttgaatactg tatcaaatga ttaatgatgg
301   cccgaatctg tatctgtatt ttttatatgc aaaatggaaa aatcgatatg
351   aatttcttcc gttatggaag aaagaatcaa atcaactatt cattcatcaa
401   atcattcact ccaaagtctg atagatcttt taaagatctg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aagaggaaaa tccgtcgact ttaaaaatcg tgagggttca
551   agtccctcta tcccc

View other 1 match with an identical amplicon

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details