JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621121.1
Database:	nt	Go to original record
Description:	Stilbe rupestris chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial)
Taxon:	Stilbe rupestris
Original size:	1628 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	667	1227	+	561 bp

Amplicon:

561 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgaaactttc aaattcagag aaaccccgga attaataaaa
101   atgggcaatc ctgagccaaa tcctgttttc tcaaaacaaa ggttcagaaa
151   acaaaaaaaa aaggataggt gcagagactc aatggaagct gttctaacaa
201   atggagttga ctgcgttggt agaggaatct ttccatcgaa acttcagaaa
251   ggatgaagga taaacgtatt tattgaatac tatatcaaat cattaatgat
301   ggcccgaatc tgtatctgta tttttttata tgaaaaatgg aagaattggt
351   gtgaattgat tccacattga aaaaaaatcg aatattcatt catcaaatca
401   ttcactccat agtctgataa atcttttaaa gaactgatta atcggacgag
451   aataaagata gagtcccatt ctacatgtca ataccggcaa caatgaaatt
501   tatagtaaaa ggaaaatccg tcgactttat aaatcgtgag ggttcaagtc
551   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	561	542	2.53e-08

Match details