JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621120.1
Database:	nt	Go to original record
Description:	Stilbe ericoides chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial)
Taxon:	Stilbe ericoides
Original size:	1281 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	309	878	+	570 bp

Amplicon:

570 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtt ggtagaggaa tctttccatc aaaacttcag
251   aaaggatgaa ggataaacgt atttattgaa tactatatca aatcattaat
301   gatggcccga atcagtatct gtattttttt atatgaaaaa tggaagaatt
351   ggtgtgaatt gatattgatt ccacattaaa aaaaaatcga atattcattc
401   atcaaatcat tcactccata gtctgataga tcttttaaag aactgattaa
451   tcggacgaga ataaagatag agtcccattc tacatgtcaa taccggcaac
501   aatgaaattt atagtaaaag gaaaatccgt cgactttata aatcgtgagg
551   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.57e-08
primerB	100.0	20 bp	0	0	570	551	2.57e-08

Match details