JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621118.1
Database:	nt	Go to original record
Description:	Nuxia oppositifolia chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial), specimen voucher Raymond 211 (UPS)
Taxon:	Nuxia oppositifolia
Original size:	1478 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	711	1273	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ctgtatctct atttttttat atgaaaaatg gaagaattgg
351   tgtgaattga tttcacattg aagaaagaat cgaatattca ttcatcaaat
401   cattcactcc atagtctgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt ataaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details