JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621116.1
Database:	nt	Go to original record
Description:	Nuxia floribunda chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial)
Taxon:	Nuxia floribunda
Original size:	1625 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	677	1241	+	565 bp

Amplicon:

565 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaaggata ggtgcagaga ctcaatggaa gctgttctaa
201   caaatggagt tgactgcgtt ggtagaggaa tctttccatc gaaacttcag
251   aaaggatgaa gaataaacgt atctattgaa tactatatca aatgattaat
301   gatggcccga atctgtatct ctattttttt atatgaaaaa tggaagaatt
351   ggtgtgaatt gattccacat tgaagaaaga atcgaatatt cattcatcaa
401   atcattcact ccatagtctg atagatcttt taaagaactg attaatcgga
451   cgagaataaa gatagagtcc cattctacat gtcaataccg gcaacaatga
501   aatttatagt aaaaggaaaa tccgtcgact ttataaatcg tgagggttca
551   agtccctcta tcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	565	546	2.54e-08

Match details