JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621115.1
Database:	nt	Go to original record
Description:	Nuxia congesta chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial), specimen voucher Bremer et al. 4260 (UPS)
Taxon:	Nuxia congesta
Original size:	1676 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	733	1295	+	563 bp

Amplicon:

563 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattataaaa
101   atgggcaatc ctgagccaaa tcctgttttc tcaaaacaaa ggttcagaaa
151   acgaaaaaaa aaaggatagg tgcagagact caatggaagc tgttctaaca
201   aatggagttg actgcgttgg tagaggaatc tttccatcga aacttcagaa
251   aggatgaagg ataaacgtat ctattgaata ctatatcaaa tgattaatga
301   tggcccgaat ctgtatctct atttttttat atgaaaaatg gaagaattgg
351   tgtgaattga ttccacattg aagaaagaat cgaatattca ttcatcaaat
401   cattcactcc atagtctgat agatctttta aagaactgat taatcggacg
451   agaataaaga tagagtccca ttctacatgt caataccggc aacaatgaaa
501   tttatagtaa aaggaaaatc cgtcgacttt ataaatcgtg agggttcaag
551   tccctctatc ccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.53e-08
primerB	100.0	20 bp	0	0	563	544	2.53e-08

Match details