JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621114.1
Database:	nt	Go to original record
Description:	Nuxia congesta chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial), specimen voucher Gerau 5231 (UPS)
Taxon:	Nuxia congesta
Original size:	1689 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	736	1299	+	564 bp

Amplicon:

564 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatcg aaacttcaga
251   aaggatgaag gataaacgta tctattgaat actatatcaa atgattaatg
301   atggcccgaa tctgtatctc tattttttta tatgaaaaat ggaagaattg
351   gtgtgaattg attccacatt gaagaaagaa tcgaatattc attcatcaaa
401   tcattcactc catagtctga tagatctttt aaagaactga ttaatcggac
451   gagaataaag atagagtccc attctacatg tcaataccgg caacaatgaa
501   atttatagta aaaggaaaat ccgtcgactt tataaatcgt gagggttcaa
551   gtccctctat cccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.54e-08
primerB	100.0	20 bp	0	0	564	545	2.54e-08

Match details