JRC GMO-Amplicons | EURL GMFF

Record ID:	AJ621113.1
Database:	nt	Go to original record
Description:	Kogelbergia verticillata chloroplast trnT-trnL IGS (partial), tRNA-Leu gene and trnL-trnF IGS (partial)
Taxon:	Kogelbergia verticillata
Original size:	1662 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	688	1254	+	567 bp

Amplicon:

567 bp

1     cgaaatcggt agacgctacg gacttaattg gattgagcct tggtatggaa
51    acctactaag tgataacttt caaattcaga gaaaccccgg aattaataaa
101   aatgggcaat cctgagccaa atcctgtttt ctcaaaacaa aggttcagaa
151   aacgaaaaaa aaaaggatag gtgcagagac tcaatggaag ctgttctaac
201   aaatggagtt gactgcgttg gtagaggaat ctttccatca aaacttcaga
251   aaggatgaag gataaacgta tttattgaat actatatcaa atcattaatg
301   atggcccgaa tctgtatctg tatttttata tgaaaaatgg aagaattggt
351   gtgaattgat attgattcca cattgaaaaa aaatcgaata ttcattcatc
401   aaatcattca ctccatagtc tgatagatct tttaaagaac tgattaatcg
451   gacgagaata aagatagagt cccattctac atgtcaatac cggcaacaat
501   gaaatttata gtaaaaggaa aatccgtcga ctttataaat cgtgagggtt
551   caagtccctc tatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.55e-08
primerB	100.0	20 bp	0	0	567	548	2.55e-08

Match details