JRC GMO-Amplicons | EURL GMFF

Record ID:	FR667112.1
Database:	nt	Go to original record
Description:	Gymnocalycium chacoense chloroplast DNA containing trnT-trnL-trnF loci, specimen voucher HA95-990-4a
Taxon:	Gymnocalycium chacoense
Original size:	1211 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	269	888	+	620 bp

Amplicon:

620 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct gagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aatgaatcaa
101   aatgggcaat cctgagccaa ctcctttaaa aaaaaaagca aaaaataagg
151   gcaagaataa aaaaaaagga aaggtgcaga gactcaaagg aagttgttct
201   aacaaatgtg ctgtgttctt atcagaattc ttccatcaaa actccaattc
251   aaaaaaaggg taaagcatat acgtagtgaa ctatatccaa tgatgaatga
301   caacccgttt atgtatatat ataaatatct ataatcagat catatgaatc
351   tgaaatctat attcgaaaat cgataattct atctaaataa aaattgtaga
401   atctgaaatg aaaaaatgga agaattttgg ggttatgaat cgattccaag
451   ttgaaaaaag aatccaatat ggatttactc catattgaag aactgattca
501   tcggacgaga atgaagatag agtcccgttc tacatgtcaa tactgacaac
551   aaagaaattt ctagtaagag gaaaatccgt cgactttaaa aatcgtgagg
601   gttcaagtcc ctctatcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.79e-08
primerB	100.0	20 bp	0	0	620	601	2.79e-08

Match details