JRC GMO-Amplicons | EURL GMFF

Record ID:	FR667109.1
Database:	nt	Go to original record
Description:	Gymnocalycium damsii var. evae chloroplast DNA containing trnT-trnL-trnF loci, specimen voucher HA1404-7
Taxon:	Gymnocalycium anisitsii subsp. damsii
Original size:	936 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	262	803	+	542 bp

Amplicon:

542 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aatgaatcaa
101   aatgggcaat cctgagccaa ctcctttcaa aaaaaagcaa aaaagaaggg
151   ttcagaaagc aagaatcaaa gaaaaggaaa ggtgcagaga ctcaaaggaa
201   gttgttctaa caaatgaggt tgactgtgct gtgttcttat cagaattctt
251   ccatcaaaac tccaattcaa aaaaagggta aagcatatac gtagtgaact
301   atatccaatg atgaatgaca acccgtttat gtatatatct aactatatat
351   aatcagataa tatgaaacaa tagataattc tatcgattcg aatctgaaat
401   gaaaaaatgg aagaattttg aatcggacga gaatgaagat agagtcccgt
451   tctacatgtc aatactgaca acaaagaaat ttcgagtaag aggaaaatcc
501   gtcgactttt aaaatcgtga gggttcaagt ccctctatcc cc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.44e-08
primerB	100.0	20 bp	0	0	542	523	2.44e-08

Match details