JRC GMO-Amplicons | EURL GMFF

Record ID:	FR667100.1
Database:	nt	Go to original record
Description:	Gymnocalycium eurypleurum chloroplast DNA containing trnT-trnL-trnF loci, specimen voucher HA1650-2
Taxon:	Gymnocalycium eurypleurum
Original size:	1208 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	270	880	+	611 bp

Amplicon:

611 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aatgaatcaa
101   aatgggcaat cctgagccaa ctcctttcaa aaaaaagcaa aaaagaaaag
151   caagaataaa aaaaaaggaa aggtgcagag actcaaagga agttgttcta
201   acaaatgagg ttgactgtgc tgtgttctta tcagaattct tccatcaaaa
251   ctccaattca aaaaaagggt aaagcatata cgtagtgaac tatatccaat
301   gatgaatgac aacccgttta tgtatatata taaatatata taatcagata
351   atatgaaaaa atagataatt ctatcgaata gaatctgaaa tgaaaaaatg
401   gaagaatttt ggggttctga atcgattcca agttgaaaaa agaatccaat
451   attgatttac tccatagtct gatatttgaa gaactgatga atcggacgag
501   aatgaagata gagtcccgtt ctacatgtca atactgacaa caaagaaatt
551   tctagtaaga ggaaaatccg tcgactttta aaatcgtgag ggttcaagtc
601   cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.75e-08
primerB	100.0	20 bp	0	0	611	592	2.75e-08

Match details