JRC GMO-Amplicons | EURL GMFF

Record ID:	FR667098.1
Database:	nt	Go to original record
Description:	Gymnocalycium monvillei subsp. horridispinum chloroplast DNA containing trnT-trnL-trnF loci, specimen voucher HT47
Taxon:	Gymnocalycium monvillei subsp. horridispinum
Original size:	1051 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	269	874	+	606 bp

Amplicon:

606 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aatgaatcaa
101   aatgggcaat cctgagccaa ctccttttaa aaaaaaagca aaaaagaaaa
151   gcaagaataa aaaaaaagga aaggtgcaga gactcaaagg aagttgttct
201   aacaaatgag gttgactgtg ctgtgttctt atcagaattc ttccatcaaa
251   actccaatta aaaaaaaggg taaagcatat acgtagtgaa ctatatccaa
301   tgattcatga caacccgttt atgtatctat atttatatat agaatcagat
351   aatatgaatc tgaaatctat attcgaaaat agagaattct atctaaataa
401   aaattgtaga atctgaaatg aaaaaatgga agaattttgg gaatcgattc
451   caagttgaaa aaagtgatag atcttttgaa gatgaatcgg acgagaatga
501   agatagagtc ccgttctaca tgtcaatact gacaacaaag aaatttctag
551   taagaggaaa atccgtcgac tttcaaaatc gtgagggttc aagtccctct
601   atcccc

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.73e-08
primerB	100.0	20 bp	0	0	606	587	2.73e-08

Match details