JRC GMO-Amplicons | EURL GMFF

Record ID:	FR667080.1
Database:	nt	Go to original record
Description:	Gymnocalycium marsoneri chloroplast DNA containing trnT-trnL-trnF loci, specimen voucher HA 1851-1
Taxon:	Gymnocalycium marsoneri
Original size:	1219 bp

Method:	QL-PLN-00-007	Go to GMOMETHODS record
		View in JRC GMO-Matrix
Description:	Qualitative PCR method for detection of chloroplast tRNA-Leu intron (ISO/FDIS 21569:2005).
Target:	chloroplast tRNA-Leu (trnL) intron

View other 20492 matches for this method

Predicted amplification details:

Mismatches	Gaps	From	To	Strand	Amplicon size
0	0	271	891	+	621 bp

Amplicon:

621 bp

1     cgaaatcggt agacgctacg gacttaattg gcttgagcct tagtatggaa
51    acctactaag tgaaaacttt caaattcaga gaaaccctgg aatgaatcaa
101   aatgggcaat cctgagccaa ctcctttcaa aaaaaagcaa aaaagaaaag
151   caagaataaa aaaaaaggaa aggtgcagag actcaaagga agttgttcta
201   acaaatgagg ttgactgtgc tgtgttctta tcataattct tccatcaaaa
251   ctccaattca aaaaaagggt aaagcatata cgtagtgaac tatatccaat
301   gatgaatgac aacccgttta tgtatatata taaatatata taatcagata
351   atatgaaaaa atagataatt ctatcgaaat caaaatttta gaatctgaaa
401   tgaaaaaatg gaagaatttt ggggttctga atcgattcca agttgaaaaa
451   agaatccaat attgatttac tccatagtct gatatttgaa gaactgatga
501   atcggacgag aatgaagata gagtcccgtt ctacatgtca atactgacaa
551   caaagaaatt tctagtaaga ggaaaatccg tcgactttta aaatcgtgag
601   ggttcaagtc cctctatccc c

Primers alignment:

Primer/Probe	% Identity	Length	Mismatches	Gaps	From	To	Score
primerA	100.0	20 bp	0	0	1	20	2.80e-08
primerB	100.0	20 bp	0	0	621	602	2.80e-08

Match details